{"id":3852,"date":"2023-05-26T10:36:22","date_gmt":"2023-05-26T16:36:22","guid":{"rendered":"https:\/\/sites.library.ualberta.ca\/ior-2020\/?page_id=3852"},"modified":"2023-05-29T10:15:20","modified_gmt":"2023-05-29T16:15:20","slug":"tripeptide-irw","status":"publish","type":"page","link":"https:\/\/sites.library.ualberta.ca\/ior-2020\/tripeptide-irw","title":{"rendered":"Tripeptide IRW Improves AMPK\/eNOS Signaling Pathway via Activating ACE2 in the Aorta of High-fat-Diet-Fed C57BL\/6 Mice"},"content":{"rendered":"\n

Submission 2023<\/h4>\n\n\n\n
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Submitted by:<\/td>Fatemeh Ashkar<\/td><\/tr>
Department:<\/td>Agricultural, Food & Nutritional Science<\/td><\/tr>
Faculty:<\/td>Agricultural, Life & Environmental Sciences<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

This study aims to investigate the effect of tripeptide IRW on the local renin\u2013angiotensin system (RAS), particularly angiotensin-converting enzyme 2 (ACE2), and their association with signaling pathways in the aorta of a high-fat-diet (HFD)-induced insulin-resistant mouse model. C57BL\/6 mice were fed HFD (45% of the total calories) for six weeks, and then IRW was added to the diet (45 mg\/kg body weight (BW)) for another eight weeks. ACE2 mRNA expression and protein level(s) were increased, while angiotensin II receptor (AT1R) and angiotensin-converting enzyme (ACE) protein abundance was significantly reduced in the aorta of HFD mice treated by IRW. IRW supplementation also improved glucose transporter 4 (GLUT4) abundance alongside AMP-activated protein kinase (AMPK), Sirtuin 1 (SIRT1), and endothelial nitric oxide synthase (eNOS) expression. IRW downregulated the levels of endothelin 1 (ET-1) and p38 mitogen-activated protein kinases (p38 MAPK). In conclusion, this study provided new evidence of the regulatory role of IRW on the aortic ACE2 against metabolic syndrome in an HFD-induced insulin-resistant model. <\/p>\n\n\n\n

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Submission 2023 Submitted by: Fatemeh Ashkar Department: Agricultural, Food & Nutritional Science Faculty: Agricultural, Life & Environmental Sciences This study aims to investigate the effect of tripeptide IRW on the local renin\u2013angiotensin system (RAS), particularly angiotensin-converting enzyme 2 (ACE2), and their association with signaling pathways in the aorta of a high-fat-diet (HFD)-induced insulin-resistant mouse model. […]<\/p>\n","protected":false},"author":3,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"class_list":["post-3852","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/sites.library.ualberta.ca\/ior-2020\/wp-json\/wp\/v2\/pages\/3852","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.library.ualberta.ca\/ior-2020\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sites.library.ualberta.ca\/ior-2020\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sites.library.ualberta.ca\/ior-2020\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.library.ualberta.ca\/ior-2020\/wp-json\/wp\/v2\/comments?post=3852"}],"version-history":[{"count":8,"href":"https:\/\/sites.library.ualberta.ca\/ior-2020\/wp-json\/wp\/v2\/pages\/3852\/revisions"}],"predecessor-version":[{"id":4288,"href":"https:\/\/sites.library.ualberta.ca\/ior-2020\/wp-json\/wp\/v2\/pages\/3852\/revisions\/4288"}],"wp:attachment":[{"href":"https:\/\/sites.library.ualberta.ca\/ior-2020\/wp-json\/wp\/v2\/media?parent=3852"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}