{"id":4730,"date":"2024-05-10T09:39:10","date_gmt":"2024-05-10T15:39:10","guid":{"rendered":"https:\/\/sites.library.ualberta.ca\/ior-2020\/?page_id=4730"},"modified":"2024-05-19T15:08:09","modified_gmt":"2024-05-19T21:08:09","slug":"more-than-meets-the-eye-actin-in-the-zebrafish-retina-2","status":"publish","type":"page","link":"https:\/\/sites.library.ualberta.ca\/ior-2020\/submissions-2024\/more-than-meets-the-eye-actin-in-the-zebrafish-retina-2","title":{"rendered":"More than meets the eye: Actin in the zebrafish retina"},"content":{"rendered":"\n

Submission 2024<\/h4>\n\n\n\n
\n
\n
\"Confocal<\/a><\/figure>\n<\/div>\n\n\n\n
\n
Submitted by:<\/td>Masha Masha<\/td><\/tr>
Department:<\/td>Cell Biology<\/td><\/tr>
Faculty:<\/td>Medicine & Dentistry<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

My research focuses on finger-like protrusions around the sensory ending of photoreceptors, the primary light-sensing cells of the retina. As those protrusions have a core of common structural protein called actin, I usually visualize them by staining the tissue with the actin-labeling dyes. This image depicts a transverse section through a transgenic Tg(sws1:GFP) zebrafish retina (UV-sensitive cone photoreceptors in magenta), demonstrating the distribution of phalloidin-stained actin (green) in the photoreceptor layer. 3D reconstruction of a confocal stack.<\/p>\n\n\n\n

Was your image created using Generative AI?<\/em>
No.<\/p>\n\n\n\n

How was your image created?
Zebrafish at 1 month post-fertilization were euthanized and fixed in paraformaldehyde, then washed and prepared for freezing using sucrose. The fish were then placed into molds and frozen in a special medium. Afterwards, I cut the block on a cryostat and collected sections on a slide. I performed a standard immunohistochemistry protocol on those slides and mounted them. Later, I imaged the slides on a confocal microscope and used Imaris software to create a 3D reconstruction.<\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Submission 2024 Submitted by: Masha Masha Department: Cell Biology Faculty: Medicine & Dentistry My research focuses on finger-like protrusions around the sensory ending of photoreceptors, the primary light-sensing cells of the retina. As those protrusions have a core of common structural protein called actin, I usually visualize them by staining the tissue with the actin-labeling […]<\/p>\n","protected":false},"author":3,"featured_media":0,"parent":4947,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"class_list":["post-4730","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/sites.library.ualberta.ca\/ior-2020\/wp-json\/wp\/v2\/pages\/4730","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.library.ualberta.ca\/ior-2020\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sites.library.ualberta.ca\/ior-2020\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sites.library.ualberta.ca\/ior-2020\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.library.ualberta.ca\/ior-2020\/wp-json\/wp\/v2\/comments?post=4730"}],"version-history":[{"count":6,"href":"https:\/\/sites.library.ualberta.ca\/ior-2020\/wp-json\/wp\/v2\/pages\/4730\/revisions"}],"predecessor-version":[{"id":5234,"href":"https:\/\/sites.library.ualberta.ca\/ior-2020\/wp-json\/wp\/v2\/pages\/4730\/revisions\/5234"}],"up":[{"embeddable":true,"href":"https:\/\/sites.library.ualberta.ca\/ior-2020\/wp-json\/wp\/v2\/pages\/4947"}],"wp:attachment":[{"href":"https:\/\/sites.library.ualberta.ca\/ior-2020\/wp-json\/wp\/v2\/media?parent=4730"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}